EE563
Selected Topics in Electrical Engineering: Flow Cytometry Data Analysis
Meeting times : Friday 13:30, 14:30, 15:30
Instructor : Bilge Karaçalı, PhD
Office : EEE Building Room 209
Phone : 6534
E-mail : bilgekaracali@iyte.edu.tr
Summary
Principles of flow cytometry; Cell preparation; Fluorochromes and fluorescence; Experimental design and fluorescence quantitation; Cell sorting; Compensation; Statistical analysis: Probability binning; Data analysis using machine learning. Readings on flow cytometry data analysis.
Grading
Class Participation 50%
Project 50%
Material
Week 1: Introduction to flow cytometry
Week 2: Fluorochromes and fluorescence
Week 3: Experimental design and fluorescence quantitation
Week 4: Compensation and gating
Week 6: Comparing Univariate Cell Distributions
Week 8: Readings on flow cytometry data analysis
Week 9: Readings on flow cytometry data analysis
Week 10: Readings on flow cytometry data analysis
Week 11: Readings on flow cytometry data analysis
Week 12: Readings on flow cytometry data analysis
Week 13: Readings on flow cytometry data analysis
Week 14: Overview